Chitosan Based Microspheres of Glibenclamide Incorporated in in-situ Gel for Nasal Delivery: Optimization and in-vitro Evaluation

The purpose of research work was to develop and optimize mucoadhesive microspheres of glibenclamide incorporated in in-situ gel for nasal delivery with the aim to enhance the residence time and improve therapeutic efficacy. Chitosan (mucoadhesive) based microspheres of glibenclamide were prepared by emulsificationcrosslinking method. Glutaraldehyde was used as crosslinking agent. A 32 factorial design was employed for preparation of microspheres wherein the concentration of polymer and crosslinking agent were selected as independent variables while particle size of microspheres, in vitro mucoadhesion and % cumulative drug permeation were the dependent variables. Microspheres were evaluated with respect to the production yield, particle size, entrapment efficiency, swelling property, in vitro mucoadhesion and % cumulative drug release. Formulation F8 was found to be optimized. The optimized formulation F8 was then formulated into thermo reversible in-situ gel. The prepared micro particulate in-situ nasal gel was mucoadhesive in nature which adhere onto the mucus and increase the residence time within the nasal cavity.